Comparative effects of Cd2+ and Cd-metallothionein on cultured kidney tubule cells. Toxicol Appl Pharmacol 1996 Feb;136(2):220-8
Date
02/01/1996Pubmed ID
8619229DOI
10.1006/taap.1996.0028Scopus ID
2-s2.0-0029664695 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
The effects of Ca2(+) and Cd-metallothionein on two cultured cells with proximal tubule characteristics, mouse kidney cortical cells and pig kidney LLC-PK1 cells, have been compared. Cd2+ inhibits Na(+)-glucose cotransport in LLC-PK1 cells and in the process decreases the number of binding sites for [3H]phlorizin, a competitive inhibitor of glucose for the Na(+)-glucose cotransporter. During 24 hr incubation and over a range of concentrations in the two cell types, only Cd2+ inhibited Na(+)-glucose cotransport even when approximately equal concentrations of intracellular Cd resulted from these treatments. Indeed, at low concentrations of Cd-metallothionein in mouse cells, transporter activity was elevated. Extension of incubations to 72 hr in mouse cells led to increased Cd uptake and reduction in cell density with both sources of Cd but only a progressive decline in Na(+)-glucose cotransport activity with Cd2+. Zn-metallothionein was without effect under comparable conditions. Both forms of Cd were accumulated by these cells, with the large majority of the metal ion localizing in metallothionein as a Cd, Zn-protein in LLC-PK1 cells. Under equal exposure conditions, the net uptake of Cd from Cd-metallothionein in the two cell types. It is evident that the mechanisms of toxicity of Cd2+ and Cd-metallothionein as well as their modes of uptake differ in these two cell types.
Author List
Blumenthal S, Lewand D, Krezoski SK, Petering DHAuthor
Samuel S. Blumenthal MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCadmium
Cell Count
Cell Line
Cell Membrane
Cell Survival
Cells, Cultured
Cytosol
Glucose
Kidney Tubules, Proximal
Metallothionein
Mice
Monosaccharide Transport Proteins
Sodium
Swine
Time Factors
