Faculty Collaboration Database

Transient repetitive exposure to low level light therapy enhances collateral blood vessel growth in the ischemic hindlimb of the tight skin mouse. Photochem Photobiol 2013;89(3):709-13

Date

12/13/2012

Pubmed ID

23231468

Pubmed Central ID

PMC3597754

DOI

10.1111/php.12024

Scopus ID

2-s2.0-84876786479 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

The tight skin mouse (Tsk(-/+)) is a model of scleroderma characterized by impaired vasoreactivity, increased oxidative stress, attenuated angiogenic response to VEGF and production of the angiogenesis inhibitor angiostatin. Low-level light therapy (LLLT) stimulates angiogenesis in myocardial infarction and chemotherapy-induced mucositis. We hypothesize that repetitive LLLT restores vessel growth in the ischemic hindlimb of Tsk(-/+) mice by attenuating angiostatin and enhancing angiomotin effects in vivo. C57Bl/6J and Tsk(-/+) mice underwent ligation of the femoral artery. Relative blood flow to the foot was measured using a laser Doppler imager. Tsk(-/+) mice received LLLT (670 nm, 50 mW cm(-2), 30 J cm(-2)) for 10 min per day for 14 days. Vascular density was determined using lycopersicom lectin staining. Immunofluorescent labeling, Western blot analysis and immunoprecipitation were used to determine angiostatin and angiomotin expression. Recovery of blood flow to the ischemic limb was reduced in Tsk(-/+) compared with C57Bl/6 mice 2 weeks after surgery. LLLT treatment of Tsk(-/+) mice restored blood flow to levels observed in C57Bl/6 mice. Vascular density was decreased, angiostatin expression was enhanced and angiomotin depressed in the ischemic hindlimb of Tsk(-/+) mice. LLLT treatment reversed these abnormalities. LLLT stimulates angiogenesis by increasing angiomotin and decreasing angiostatin expression in the ischemic hindlimb of Tsk(-/+) mice.

Author List

Zaidi M, Krolikowki JG, Jones DW, Pritchard KA Jr, Struve J, Nandedkar SD, Lohr NL, Pagel PS, Weihrauch D

Authors

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin
Dorothee Weihrauch DVM, PhD Research Scientist II in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Angiostatins
Animals
Capillaries
Disease Models, Animal
Femoral Artery
Gene Expression Regulation
Hindlimb
Intercellular Signaling Peptides and Proteins
Ischemia
Ligation
Light
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microfilament Proteins
Neovascularization, Physiologic
Recovery of Function
Scleroderma, Systemic