Occult tumour cells in peritoneal lavage are a negative prognostic factor in pancreatic cancer. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2013 Sep;157(3):233-8
Date
11/07/2012Pubmed ID
23128819DOI
10.5507/bp.2012.061Scopus ID
2-s2.0-84887310065 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
AIMS: The aim of this study was to test the hypothesis that occult tumour cells in peritoneal lavage are a negative prognostic factor in pancreatic adenocarcinoma.
METHODS: Real-time RT-PCR analysis of CEA, EGFR and hTERT transcript levels was used to identify occult tumour cells in peritoneal lavage samples from 96 pancreatic cancer patients.
RESULTS: We found significant association between CEA expression levels in peritoneal lavage and clinical stage. We also found that EGFR transcript levels were higher in peritoneal lavage samples from patients with high grade tumours than in samples from patients with low grade tumours. Detection of CEA and/or EGFR occult tumour cell markers in the peritoneal lavage was associated with significantly shorter overall survival and increased hazard ratio for disease recurrence.
CONCLUSIONS: The results show that the presence of occult tumour cells in peritoneal lavage is a negative prognostic factor for survival in pancreatic cancer patients, and that detection of occult tumour cells using PCR-based methods can identify patients with advanced disease for whom radical surgery is likely to have little benefit.
Author List
Havlik R, Srovnal J, Klos D, Benedikova A, Lovecek M, Ghothim M, Cahova D, Neoral C, Hajduch MAuthor
Robert Havlik MD Chair, Professor in the Plastic Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdenocarcinomaAdult
Aged
Aged, 80 and over
Ascitic Fluid
Biomarkers, Tumor
Carcinoembryonic Antigen
Disease-Free Survival
ErbB Receptors
Female
Humans
Male
Middle Aged
Neoplasm Recurrence, Local
Pancreatic Neoplasms
Prognosis
Telomerase
