Faculty Collaboration Database

Postprandial total and HMW adiponectin following a high-fat meal in lean, obese and diabetic men. Eur J Clin Nutr 2013 Apr;67(4):377-84

Date

03/07/2013

Pubmed ID

23462948

DOI

10.1038/ejcn.2013.49

Scopus ID

2-s2.0-84876417126 (requires institutional sign-in at Scopus site)   20 Citations

Abstract

BACKGROUND/OBJECTIVES: Recent work suggests that macronutrients are pro-inflammatory and promote oxidative stress. Reports of postprandial regulation of total adiponectin have been mixed, and there is limited information regarding postprandial changes in high molecular weight (HMW) adiponectin. The aim of this study was to assess the effect of a standardised high-fat meal on metabolic variables, adiponectin (total and HMW), and markers of inflammation and oxidative stress in: (i) lean, (ii) obese non-diabetic and (iii) men with type 2 diabetes mellitus (T2DM).

SUBJECTS/METHODS: Male subjects: lean (n=10), obese (n=10) and T2DM (n=10) were studied for 6 h following both a high-fat meal and water control. Metabolic variables (glucose, insulin, triglycerides), inflammatory markers (interleukin-6 (IL6), tumour necrosis factor (TNF)α, high-sensitivity C-reactive protein (hsCRP), nuclear factor (NF)κB expression in peripheral blood mononuclear cells (p65)), indicators of oxidative stress (oxidised low density lipoprotein (oxLDL), protein carbonyl) and adiponectin (total and HMW) were measured.

RESULTS: No significant changes in TNFα, p65, oxLDL or protein carbonyl concentrations were observed. Overall, postprandial IL6 decreased in subjects with T2DM but increased in lean subjects, whereas hsCRP decreased in the lean cohort and increased in obese subjects. There was no overall postprandial change in total or HMW adiponectin in any group. Total adiponectin concentrations changed over time following the water control, and the response was significantly different in lean subjects compared with subjects with T2DM (P=0.04).

CONCLUSIONS: No consistent significant postprandial inflammation, oxidative stress or regulation of adiponectin was observed in this study. Findings from the water control suggest differential basal regulation of total adiponectin in T2DM compared with lean controls.

Author List

Phillips LK, Peake JM, Zhang X, Hickman IJ, Briskey DR, Huang BE, Simpson P, Li SH, Whitehead JP, Martin JH, Prins JB

Author

Pippa M. Simpson PhD Adjunct Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adiponectin
Adult
Aged
Biomarkers
Blood Glucose
C-Reactive Protein
Diabetes Mellitus, Type 2
Diet, High-Fat
Humans
Inflammation
Insulin
Interleukin-6
Leukocytes, Mononuclear
Lipoproteins, LDL
Male
Meals
Middle Aged
Molecular Weight
NF-kappa B
Obesity
Oxidative Stress
Postprandial Period
Thinness
Triglycerides
Tumor Necrosis Factor-alpha