Faculty Collaboration Database

Visualizing the prostate gland by MR imaging in young and old mice. PLoS One 2013;8(3):e55746

Date

03/08/2013

Pubmed ID

23469167

Pubmed Central ID

PMC3585879

DOI

10.1371/journal.pone.0055746

Scopus ID

2-s2.0-84874559718 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

PURPOSE: Prostate imaging requires optimization in young and old mouse models. We tested which MR sequences and field strengths best depict the prostate gland in young and old mice; and, whether prostate MR signal, size, and architecture change with age.

TECHNIQUE: Magnetic resonance imaging (MRI) of the prostate of young (2 months) and old (18 months) male nude mice (n = 6) was performed at 4.7 and 7 T and SCID mice (n = 6) at 7 T field strengths, using T1, fat suppressed T1, DWI, T2, fat suppressed T2, as well as T2-based- and proton density-based Dixon "water only" sequences. Images were ranked for best overall sequence for prostate visualization, prostate delineation, and quality of fat suppression. Prostate volume and signal characteristics were compared and histology was performed.

RESULTS: T2-based-Dixon "water only" images ranked best overall for prostate visualization and delineation as well as fat suppression (n = 6, P<0.001) at both 4.7 T and 7 T in nude and 7T in SCID mice. Evaluated in nude mice, T2-based Dixon "water only" had greater prostate CNR and lower fat SNR at 7 T than 4.7 T (P<0.001). Prostate volume was less in older than younger mice (n = 6, P<0.02 nude mice; n = 6, P<0.002 SCID mice). Prostate T2 FSE as well as proton density-based and T2-based-Dixon "water only" signal intensity was higher in younger than older mice (P<0.001 nude mice; P<0.01 SCID mice) both at 4.7 and 7 T. This corresponded to an increase in glandular hyperplasia in older mice by histology (P<0.01, n = 6).

CONCLUSION: T2-based Dixon "water only" images best depict the mouse prostate in young and old nude mice at 4.7 and 7 T. The mouse prostate decreases in size with age. The decrease in T2 and T2-based Dixon "water only" signal with age corresponds with glandular hyperplasia. Findings suggest age should be an important determinant when choosing models of prostate biology and disease.

Author List

Ravoori M, Duggal J, Gagea M, Han L, Singh S, Liu P, Wei W, Ragan DK, Bankson JA, Ma J, Kundra V

Author

Dustin K. Ragan PhD Assistant Professor in the Radiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Age Factors
Animals
Histocytochemistry
Intra-Abdominal Fat
Magnetic Resonance Imaging
Male
Mice
Mice, Nude
Mice, SCID
Organ Size
Prostate
Prostatic Hyperplasia