RNA localization in yeast: moving towards a mechanism. Biol Cell 2005 Jan;97(1):75-86
Date
12/17/2004Pubmed ID
15601259DOI
10.1042/BC20040066Scopus ID
2-s2.0-13544261683 (requires institutional sign-in at Scopus site) 100 CitationsAbstract
RNA localization is a widely utilized strategy employed by cells to spatially restrict protein function. In Saccharomyces cerevisiae asymmetric sorting of mRNA to the bud has been reported for at least 24 mRNAs. The mechanism by which the mRNAs are trafficked to the bud, illustrated by ASH1 mRNA, involves recognition of cis-acting localization elements present in the mRNA by the RNA-binding protein, She2p. The She2p/mRNA complex subsequently associates with the myosin motor protein, Myo4p, through an adapter, She3p. This ribonucleoprotein complex is transported to the distal tip of the bud along polarized actin cables. While the mechanism by which ASH1 mRNA is anchored at the bud tip is unknown, current data point to a role for translation in this process, and the rate of translation of Ash1p during the transport phase is regulated by the cis-acting localization elements. Subcellular sorting of mRNA in yeast is not limited to the bud; certain mRNAs corresponding to nuclear-encoded mitochondrial proteins are specifically sorted to the proximity of mitochondria. Analogous to ASH1 mRNA localization, mitochondrial sorting requires cis-acting elements present in the mRNA, though trans-acting factors involved with this process remain to be identified. This review aims to discuss mechanistic details of mRNA localization in S. cerevisiae.
Author List
Gonsalvez GB, Urbinati CR, Long RMAuthor
Roy M. Long PhD Assistant Dean, Professor in the Medical School Regional Campuses department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell PolarityCytoskeleton
DNA-Binding Proteins
Mitochondria
Protein Biosynthesis
RNA, Messenger
Repressor Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
