Properties of membranes derived from the total lipids extracted from the human lens cortex and nucleus. Biochim Biophys Acta 2013 Jun;1828(6):1432-40
Date
02/27/2013Pubmed ID
23438364Pubmed Central ID
PMC3633468DOI
10.1016/j.bbamem.2013.02.006Scopus ID
2-s2.0-84874941366 (requires institutional sign-in at Scopus site) 52 CitationsAbstract
Human lens lipid membranes prepared using a rapid solvent exchange method from the total lipids extracted from the clear lens cortex and nucleus of 41- to 60-year-old donors were investigated using electron paramagnetic resonance spin-labeling. Profiles of the phospholipid alkyl-chain order, fluidity, oxygen transport parameter, and hydrophobicity were assessed across coexisting membrane domains. Membranes prepared from the lens cortex and nucleus were found to contain two distinct lipid environments, the bulk phospholipid-cholesterol domain and the cholesterol bilayer domain (CBD). The alkyl chains of phospholipids were strongly ordered at all depths, indicating that the amplitude of the wobbling motion of alkyl chains was small. However, profiles of the membrane fluidity, which explicitly contain time (expressed as the spin-lattice relaxation rate) and depend on the rotational motion of spin labels, show relatively high fluidity of alkyl chains close to the membrane center. Profiles of the oxygen transport parameter and hydrophobicity have a rectangular shape and also indicate a high fluidity and hydrophobicity of the membrane center. The amount of CBD was greater in nuclear membranes than in cortical membranes. The presence of the CBD in lens lipid membranes, which at 37°C showed a permeability coefficient for oxygen about 60% smaller than across a water layer of the same thickness, would be expected to raise the barrier for oxygen transport across the fiber cell membrane. Properties of human membranes are compared with those obtained for membranes made of lipids extracted from cortex and nucleus of porcine and bovine eye lenses.
Author List
Mainali L, Raguz M, O'Brien WJ, Subczynski WKAuthor
Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultCell Membrane
Cell Membrane Permeability
Diffusion
Electron Spin Resonance Spectroscopy
Humans
Hydrophobic and Hydrophilic Interactions
Lens Cortex, Crystalline
Lens Nucleus, Crystalline
Liposomes
Membrane Fluidity
Membrane Lipids
Middle Aged
Molecular Structure
Oxygen
Phospholipids
Time Factors
