The effect of calcium modulating agents on peripheral nerve recovery after crush. J Neurosci Methods 2013 Jul 15;217(1-2):54-62
Date
05/01/2013Pubmed ID
23628158DOI
10.1016/j.jneumeth.2013.04.013Scopus ID
2-s2.0-84878423103 (requires institutional sign-in at Scopus site) 9 CitationsAbstract
After a nerve injury, calcium concentration in the intra-nerve fiber drastically increases. The purpose of our study was to test an implantable micro-osmotic pump to deliver medications to accelerate calcium absorption, thereby greatly improving nerve regeneration. Twenty-four SD rats were divided into four groups of six each: (1) Sham control: crush injury to sciatic nerve only; (2) Crush injury with a Nifedipine pump; (3) Crush injury with a Calcitonin pump; (4) Crush injury with a Saline pump. Each rat's right sciatic nerve was crushed. The micro-osmotic pump was implanted in the neck, and the dripping tube was routed to the injured nerve. After four weeks of survival time, compound muscle action potential (CMAP), tetanic muscle force (TMF), myelinated nerve fiber area (NFA), nerve calcium concentration (NCC), and calcified spots (CS) were evaluated. The calcium absorption rate (CAR) was also determined. The order from highest to lowest recovery rate was Nifedipine>Calcitonin>Sham control>Saline. Differences among the groups were statistically significant (P<0.001, ANOVA test), and the difference between Nifedipine/Calcitonin and Saline/Sham control were all statistically significant (P<0.001, t-test). The correlation rate of NCC with CMAP/TMF and with NFA/CS and CAR were calculated to be 0.99 (all P<0.001, Pearson's Correlation). We conclude from this study that nerve regeneration strongly correlated with calcium absorption; our new data has shown greatly improved nerve functional recovery, and this can potentially be translated into clinical applications.
Author List
Yan JG, Zhang LL, Agresti M, Logiudice J, Yan YH, Wang Z, Sanger JR, Matloub HSAuthors
John A. LoGiudice MD Professor in the Plastic Surgery department at Medical College of WisconsinHani S. Matloub MD Professor in the Plastic Surgery department at Medical College of Wisconsin
James R. Sanger MD Professor in the Plastic Surgery department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCalcium
Calcium Channel Blockers
Down-Regulation
Male
Nerve Regeneration
Peripheral Nerve Injuries
Peripheral Nerves
Rats
Rats, Sprague-Dawley
Recovery of Function
Treatment Outcome
