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[Effects of hsf1 genotype on the constitutive expression of alphaB-crystallin in mice myocardium]. Zhongguo Ying Yong Sheng Li Xue Za Zhi 2004 Feb;20(1):30-3

Date

02/01/2004

Pubmed ID

21162300

Scopus ID

2-s2.0-84871946560

Abstract

AIM: Try to clarify the effects of HSF1 gene on the constitutively expressed alphaBC.

METHODS: To investigate the levels of constitutively expressed alphaB-Crystallin (alphaBC) in hsf1 knockout (hsf1 -/-) and hsf1 wild type (hsf1 +/+) mice myocardium by Western blot and immunohistochemistry.

RESULTS: The alphaBC levels in hsf1 -/- and hsf1 +/+ were 68.42% +/- 4.16%, 100% +/- 7.58%, respectively (P < 0.05, cytosolic fraction), and 20.53% +/- 1.01%, 37.55% +/- 1.91%, respectively (P < 0.05, pellet fraction). The alphaBC signals decreased significantly in hsf1 -/- myocardium compared with hsf1 +/+ myocardium stained with fluorescence immunohistochemistry.

CONCLUSION: hsf1 is the important, but not the only factor, which mediates the constitutively expressed alphaBC.

Author List

Liu L, Zhang HH, Ding GX, Cheng YL, Yan LJ, Benjamin IJ

Author

Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
DNA-Binding Proteins
Female
Genotype
Heat Shock Transcription Factors
Male
Mice
Mice, Knockout
Myocardium
Transcription Factors
alpha-Crystallin B Chain
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a