Medical College of Wisconsin
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Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation. Genes Dev 2003 Jun 01;17(11):1392-401

Date

06/05/2003

Pubmed ID

12782657

Pubmed Central ID

PMC196071

DOI

10.1101/gad.1071803

Scopus ID

2-s2.0-0038623298   108 Citations

Abstract

To understand the role of chromatin-remodeling activities in transcription, it is necessary to understand how they interact with transcriptional activators in vivo to regulate the different steps of transcription. Human heat shock factor 1 (HSF1) stimulates both transcriptional initiation and elongation. We replaced mouse HSF1 in fibroblasts with wild-type and mutant human HSF1 constructs and characterized regulation of an endogenous mouse hsp70 gene. A mutation that diminished transcriptional initiation led to twofold reductions in hsp70 mRNA induction and recruitment of a SWI/SNF remodeling complex. In contrast, a mutation that diminished transcriptional elongation abolished induction of full-length mRNA, SWI/SNF recruitment, and chromatin remodeling, but minimally impaired initiation from the hsp70 promoter. Another remodeling factor, SNF2h, is constitutively present at the promoter irrespective of the genotype of HSF1. These data suggest that localized recruitment of SWI/SNF drives a specialized remodeling reaction necessary for the production of full-length hsp70 mRNA.

Author List

Corey LL, Weirich CS, Benjamin IJ, Kingston RE

Author

Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cell Line
Chromatin
Cloning, Molecular
DNA-Binding Proteins
Fibroblasts
Gene Expression Regulation
HSP70 Heat-Shock Proteins
Heat Shock Transcription Factors
Humans
Mice
Mice, Knockout
RNA, Messenger
Recombinant Proteins
Transcription Factors
Transcription, Genetic
Transfection
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a