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Heat shock factor 1-independent activation of dendritic cells by heat shock: implication for the uncoupling of heat-mediated immunoregulation from the heat shock response. Eur J Immunol 2003 Jun;33(6):1754-62

Date

06/05/2003

Pubmed ID

12778494

DOI

10.1002/eji.200323687

Scopus ID

2-s2.0-0037744720   23 Citations

Abstract

The induction of heat shock proteins by heat shock is classically defined as the heat shock response, which is involved in cytoprotection, inflammation and immune responses. Whereas the cytoprotective properties of heat shock have been well characterized, the immunomodulating roles of the heat shock response on the immune system are just emerging. In particular, it is not known whether immunomodulating functions of heat are mediated by the heat shock response. We addressed this question genetically, using a murine model that is unable to mount the heat shock response because of deletion of a major transcriptional factor, heat shock factor 1 (Hsf1). We focused on the roles of heat shock on modulating the functions of dendritic cells (DC) because of their important roles in both innate and adaptive immunity. We found that heat shock matures CD11c(+) DC both in vitro and in vivo, phenotypically and functionally, in the absence of any exogenous inflammatory stimuli. Furthermore, heat-shock-mediated DC maturation is independent of Hsf1, as Hsf1(-/-) DC can be matured by heat shock equally well as wild-type DC. Our novel findings demonstrate that heat shock, one of the most primitive biological responses, can modulate the immune response without the requirement for the transcriptional induction/repression of target genes mediated by Hsf1.

Author List

Zheng H, Benjamin IJ, Basu S, Li Z

Author

Ivor J. Benjamin MD Center Director, Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Bone Marrow Cells
CD11c Antigen
Cells, Cultured
DNA-Binding Proteins
Dendritic Cells
Egg Proteins
Epitopes, T-Lymphocyte
Female
Heat Shock Transcription Factors
Heat-Shock Response
Hot Temperature
Hyperthermia, Induced
Male
Mice
Mice, Inbred BALB C
Mice, Knockout
Mice, Transgenic
Ovalbumin
Peptide Fragments
Receptors, Antigen, T-Cell
Transcription Factors
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a