Faculty Collaboration Database

Medullary thick ascending limb buffer vasoconstriction of renal outer-medullary vasa recta in salt-resistant but not salt-sensitive rats. Hypertension 2012 Oct;60(4):965-72

Date

08/29/2012

Pubmed ID

22926950

Pubmed Central ID

PMC3785299

DOI

10.1161/HYPERTENSIONAHA.112.195214

Scopus ID

2-s2.0-84866562683 (requires institutional sign-in at Scopus site)   18 Citations

Abstract

We have demonstrated previously that paracrine signaling occurs between medullary thick ascending limb (mTAL) and the contractile pericytes of outer-medullary vasa recta (VR), termed "tubular-vascular cross-talk." The aim of the current study was to determine whether tubular-vascular cross-talk has a functional effect on vasoconstrictor responses to angiotensin II and to determine whether this is altered in the Dahl salt-sensitive (SS) rat. Studies were performed on salt-resistant consomic SS.13 Brown Norway (BN) and SS rats using a novel outer medullary tissue strip preparation in which freshly isolated VRs within VR bundles were perfused either alone or in combination with nearby mTAL. In VRs from SS.13BN rats, angiotensin II (1 µmol/L) increased VR bundle intracellular Ca2+ concentration 19±9 nmol/L (n=8) and reduced focal diameter in perfused VRs by -20±7% (n=5). In the presence of nearby mTAL, however, VR bundle intracellular Ca2+ concentration (-9±8 nmol/L; n=8) and VR diameter (-1±4%, n=7) in SS.13BN rats were unchanged by angiotensin II. In contrast, in Dahl SS rats, angiotensin II resulted in rapid and sustained increase in VR bundle intracellular Ca2+ concentration (89±48 nmol/L, n=7; 50±24%, n=8) and a reduction in VR diameter of (-17±7%, n=7; -11±4%, n=5) in both isolated VRs and VRs with nearby mTAL, respectively. In VRs with mTAL from SS13BN rats, inhibiton of purinergic receptors resulted in an increase in VR bundle intracellular Ca2+ concentration, indicating that purinergic signaling buffers vasoconstriction. Importantly, our in vitro data were able to predict medullary blood flow responses to angiotensin II in SS and SS.13BN rats in vivo. We conclude that paracrine signaling from mTAL buffers angiotensin II vasoconstriction in Dahl salt-resistant SS.13BN rats but not SS rats.

Author List

O'Connor PM, Cowley AW Jr

Author

Allen W. Cowley Jr PhD Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Angiotensin II
Animals
Arterial Pressure
Calcium
Kidney Medulla
Male
Rats
Rats, Inbred BN
Rats, Inbred Dahl
Renal Circulation
Sodium Chloride, Dietary
Vasoconstriction
Vasoconstrictor Agents