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Partitional and motional properties of a spin-labeled daunomycin in lipid bilayers. An ESR study. J Inorg Biochem 1992 May 01;46(2):67-76

Date

05/01/1992

Pubmed ID

1326024

DOI

10.1016/0162-0134(92)80010-s

Abstract

The partition coefficient of a spin-labeled daunomycin (DAU-SL) in dimyristoylphosphatidylcholine membrane has been determined using the electron spin resonance (ESR) method. The experiment was carried out as a function of temperature between 5 degrees C and 35 degrees C, giving partition coefficients between 2 and 6 without abrupt change at the phase transition. The thermodynamic parameters on transferring the DAU-SL from the aqueous phase to the lipid bilayer were also calculated. The calculated values are: delta H = 6.11 kcal/mol and delta S = 23 cal/K mol. The partitioning of the DAU-SL and its motion in the membrane were investigated in a wide range of pH (4-10.3). The data show that pH has no effect on partitioning of the DAU-SL which suggest that the drug exists in the uncharged form in the bilayer.

Author List

Pezeshk A, Pezeshk V, Wojas J, Subczynski WK

Author

Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Chemical Phenomena
Chemistry, Physical
Daunorubicin
Dimyristoylphosphatidylcholine
Electron Spin Resonance Spectroscopy
Hydrogen-Ion Concentration
Lipid Bilayers
Liposomes
Spin Labels
Thermodynamics