Faculty Collaboration Database

Differential lung uptake of 99mTc-hexamethylpropyleneamine oxime and 99mTc-duramycin in the chronic hyperoxia rat model. J Nucl Med 2012 Dec;53(12):1984-91

Date

10/23/2012

Pubmed ID

23086010

Pubmed Central ID

PMC4406414

DOI

10.2967/jnumed.112.108498

Scopus ID

2-s2.0-84870346596 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

UNLABELLED: Noninvasive radionuclide imaging has the potential to identify and assess mechanisms involved in particular stages of lung injury that occur with acute respiratory distress syndrome, for example. Lung uptake of (99m)Tc-hexamethylpropyleneamine oxime (HMPAO) is reported to be partially dependent on the redox status of the lung tissue whereas (99m)Tc-duramycin, a new marker of cell injury, senses cell death via apoptosis or necrosis. Thus, we investigated changes in lung uptake of these agents in rats exposed to hyperoxia for prolonged periods, a common model of acute lung injury.

METHODS: Male Sprague-Dawley rats were preexposed to either normoxia (21% O(2)) or hyperoxia (85% O(2)) for up to 21 d. For imaging, the rats were anesthetized and injected intravenously with either (99m)Tc-HMPAO or (99m)Tc-duramycin (both 37-74 MBq), and planar images were acquired using a high-sensitivity modular γ-camera. Subsequently, (99m)Tc-macroagreggated albumin (37 MBq, diameter 10-40 μm) was injected intravenously, imaged, and used to define a lung region of interest. The lung-to-background ratio was used as a measure of lung uptake.

RESULTS: Hyperoxia exposure resulted in a 74% increase in (99m)Tc-HMPAO lung uptake, which peaked at 7 d and persisted for the 21 d of exposure. (99m)Tc-duramycin lung uptake was also maximal at 7 d of exposure but decreased to near control levels by 21 d. The sustained elevation of (99m)Tc-HMPAO uptake suggests ongoing changes in lung redox status whereas cell death appears to have subsided by 21 d.

CONCLUSION: These results suggest the potential use of (99m)Tc-HMPAO and (99m)Tc-duramycin as redox and cell-death imaging biomarkers, respectively, for the in vivo identification and assessment of different stages of lung injury.

Author List

Clough AV, Audi SH, Haworth ST, Roerig DL

Authors

Said Audi PhD Professor in the Biomedical Engineering department at Marquette University
Anne Clough PhD Professor in the Mathematics, Statistics, and Computer Science department at Marquette University

MESH terms used to index this publication - Major topics in bold

Animals
Bacteriocins
Biological Transport
Chronic Disease
Disease Models, Animal
Hyperoxia
Lung
Male
Oxidative Stress
Peptides
Radionuclide Imaging
Rats
Rats, Sprague-Dawley
Technetium Tc 99m Exametazime