Faculty Collaboration Database

Unique contractile and structural protein expression in dog ileal inner circular smooth muscle. J Smooth Muscle Res 2009 Oct;45(5):217-30

Date

11/13/2009

Pubmed ID

19907120

DOI

10.1540/jsmr.45.217

Scopus ID

2-s2.0-73949085718 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

This study was designed to test the hypothesis that there is heterogeneous expression of contractile and structural proteins between the smooth muscle cells (SMCs) in the inner and outer circular muscle (ICM and OCM) layers of the ileum. Immunohistochemical staining and quantitation of fresh frozen sections of the dog ileum was performed using protein specific antibodies. Smooth muscle (SM) SMA myosin heavy chain (MHC), alpha- and gamma-SM actin, and vinculin all show greater expression in the ICM relative to the OCM. SMB MHC and fibronectin show the opposite pattern, with greater expression in the OCM relative to the ICM. Differences in expression of these proteins are consistent with proposed differences in function of these muscle layers. Hypotheses regarding muscle tone and the coordination and regulation of peristalsis via these different muscle layers based on this data can now be made and tested.

Author List

Eddinger TJ

Author

Thomas Eddinger PhD Bioological Sciences in the Biology department at Marquette University

MESH terms used to index this publication - Major topics in bold

Actins
Animals
Dogs
Ileum
Immunohistochemistry
Muscle Contraction
Muscle Proteins
Muscle, Smooth
Myosin Heavy Chains
Rabbits
Vinculin