Faculty Collaboration Database

Shortening velocity and myosin heavy- and light-chain isoform mRNA in rabbit arterial smooth muscle cells. Am J Physiol Cell Physiol 2002 May;282(5):C1093-102

Date

04/10/2002

Pubmed ID

11940525

DOI

10.1152/ajpcell.00307.2001

Scopus ID

2-s2.0-0036089197 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

In smooth muscle cells (SMCs) isolated from rabbit carotid, femoral, and saphenous arteries, relative myosin isoform mRNA levels were measured in RT-PCR to test for correlations between myosin isoform expression and unloaded shortening velocity. Unloaded shortening velocity and percent smooth muscle myosin heavy chain 2 (SM2) and myosin light chain 17b (MLC(17b)) mRNA levels were not significantly different in single SMCs isolated from the luminal and adluminal regions of the carotid media. Saphenous artery SMCs shortened significantly faster (P < 0.05) than femoral SMCs and had more SM2 mRNA (P < 0.05) than carotid SMCs and less MLC(17b) mRNA (P < 0.001) and higher tissue levels of SMB mRNA (P < 0.05) than carotid and femoral SMCs. No correlations were found between percent SM2 and percent MLC(17b) mRNA levels and unloaded shortening velocity in SMCs from these arteries. We have previously shown that myosin heavy chain (MHC) SM1/SM2 and SMA/SMB and MLC(17a)/MLC(17b) isoform mRNA levels correlate with protein expression for these isoforms in rabbit smooth muscle tissues. Thus we interpret these results to suggest that 1) SMC myosin isoform expression and unloaded shortening velocity do not vary with distance from the lumen of the carotid artery but do vary in arteries located longitudinally within the arterial tree, 2) MHC SM1/SM2 and/or MLC(17a)/MLC(17b) isoform expression does not correlate with unloaded shortening velocity, and 3) intracellular expression of the MHC SM1/SM2 and MLC(17a)/MLC(17b) isoforms is not coregulated.

Author List

Sherwood JJ, Eddinger TJ

Author

Thomas Eddinger PhD Bioological Sciences in the Biology department at Marquette University

MESH terms used to index this publication - Major topics in bold

Animals
Arteries
Cells, Cultured
Female
Muscle Contraction
Muscle, Smooth, Vascular
Myosin Heavy Chains
Myosin Light Chains
Protein Isoforms
RNA, Messenger
Rabbits
Time Factors