Cyclosporin A and hydroxycyclosporine (M-17) affect the secretory phenotype of human gingival fibroblasts. J Oral Pathol Med 1998 Jul;27(6):260-6
Date
08/26/1998Pubmed ID
9707278DOI
10.1111/j.1600-0714.1998.tb01953.xScopus ID
2-s2.0-0032126051 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
The responsiveness of human gingival fibroblast populations to cyclosporin A (CsA) and its principal metabolite, hydroxycyclosporine (M17), was evaluated in cell culture. Gingival fibroblasts exhibited a dose-dependent accumulation and bell-shaped distribution of dansylated CsA. A 100-fold excess of non-labeled CsA prevented the accumulation of the fluorescent probe in the fibroblasts. Both CsA (400 ng/ml) and M17 (100 ng/ml) stimulated mean gingival fibroblast cell number to 23.2% and 36.7% above controls, and reduced mean collagen production by 37.7% and 37.4% below controls, respectively; however, neither CsA nor M17 affected mean protein production in comparison to control cultures. Analyses of responses to CsA and M17 by ligand-accumulating and non-accumulating fibroblasts sorted out from the parent cultures did not provide consistent interstrain responses either by cells representing the upper quartile of fluorescence or cells representing the bottom quartiles of fluorescence. These data demonstrate that CsA is accumulated by gingival fibroblasts and that CsA and M17 are potent modulators of gingival fibroblast phenotype.
Author List
Mariotti A, Hassell T, Jacobs D, Manning CJ, Hefti AFAuthor
Arthur Hefti DDS,PhD Associate Dean - Research & Graduate Studies in the Dentistry department at Marquette UniversityMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Aged
Analysis of Variance
Cell Count
Cell Division
Cell Separation
Cells, Cultured
Collagen
Cyclosporine
Cyclosporins
Dose-Response Relationship, Drug
Female
Fibroblasts
Flow Cytometry
Fluorescent Dyes
Gingiva
Humans
Immunosuppressive Agents
Ligands
Male
Middle Aged
Phenotype
Protein Biosynthesis
Proteins
