Antegrade iliac artery stent implantation for the temporal and spatial examination of stent-induced neointimal hyperplasia and alterations in regional fluid dynamics. J Pharmacol Toxicol Methods 2005;51(2):115-21
Date
03/16/2005Pubmed ID
15767204DOI
10.1016/j.vascn.2004.08.001Scopus ID
2-s2.0-17644425726 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
INTRODUCTION: Neointimal hyperplasia remains an important problem after stent implantation. Previous investigations examining vascular responses to stent implantation and effects of drugs have used a retrograde deployment approach that may inadvertently alter the local fluid dynamics surrounding the stent. We present a model of antegrade iliac artery stent implantation that facilitates the analysis of stent-induced alterations in neointimal hyperplasia and wall shear stress in vivo.
METHODS: Stent delivery catheters were inserted through the left carotid artery in anesthetized rabbits (n=37). Catheters were advanced under fluoroscopic guidance to the distal iliac arteries, where the stent was deployed. Hemotoxylin and eosin (H&E) staining of unstented and stented vascular sections was performed 21 days after implantation.
RESULTS: Selective unilateral stent implantation was successful in 32 of 37 rabbits. No histological abnormalities were observed in the aorta, contralateral unstented iliac, or distal femoral arteries. Neointimal hyperplasia was localized to the stented region.
DISCUSSION: The model of stent implantation was relatively easy to perform and produced selective neointimal hyperplasia within the stented region without evidence of damage, cellular proliferation, or flow disruption in the surrounding normal arterial vessels. The model will allow detailed examination of the influence of stent implantation on indices of wall shear stress, neointimal hyperplasia, the mechanisms of cellular proliferation in vivo, and their modification by drugs.
Author List
LaDisa JF Jr, Meier HT, Olson LE, Kersten JR, Warltier DC, Pagel PSAuthor
John F. LaDisa PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood Vessel Prosthesis Implantation
Hyperplasia
Iliac Artery
Male
Models, Cardiovascular
Rabbits
Radiography
Regional Blood Flow
Stents
Tunica Intima
