Histochemical discrimination of fibers in regenerating rat infraorbital nerve. Microsurgery 1992;13(1):39-44
Date
01/01/1992Pubmed ID
1375308DOI
10.1002/micr.1920130110Scopus ID
2-s2.0-0026505564 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
In rat dorsal root ganglia, histochemical staining of carbonic anhydrase (CA) and cholinesterase (CE) yields a reciprocal pattern of activity: Sensory processes are CA positive and CE negative, whereas motor processes are CA negative and CE positive. In rat infraorbital nerve (a sensory peripheral nerve), we saw extensive CA staining of nearly 100% of the myelinated axons. Although CE reactivity in myelinated axons was extremely rare, we did observe CE staining of unmyelinated autonomic fibers. Four weeks after transection of infraorbital nerves, CA-stained longitudinal sections of the proximal stump demonstrated 3 distinct morphological zones. A fraction of the viable axons retained CA activity to within 2 mm of the distal extent of the stump, and the stain is capable of resolving growth sprouts being regenerated from these fibers. Staining of unmyelinated autonomic fibers in serial sections shows that CE activity was not retained as far distally as is the CA sensory staining.
Author List
Wilke RA, Riley DA, Sanger JRAuthor
James R. Sanger MD Professor in the Plastic Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAxons
Carbonic Anhydrases
Cholinesterases
Ganglia, Spinal
Histocytochemistry
Male
Maxillary Nerve
Motor Neurons
Nerve Degeneration
Nerve Fibers
Nerve Fibers, Myelinated
Nerve Regeneration
Neurons, Afferent
Orbit
Rats
Rats, Inbred Strains
Spinal Nerve Roots
Staining and Labeling
