Transcriptional regulatory regions of gap43 needed in developing and regenerating retinal ganglion cells. Dev Dyn 2010 Feb;239(2):482-95
Date
12/25/2009Pubmed ID
20034105Pubmed Central ID
PMC3101714DOI
10.1002/dvdy.22190Scopus ID
2-s2.0-75149113267 (requires institutional sign-in at Scopus site) 33 CitationsAbstract
Mammals and fish differ in their ability to express axon growth-associated genes in response to CNS injury, which contributes to the differences in their ability for CNS regeneration. Previously we demonstrated that for the axon growth-associated gene, gap43, regions of the rat promoter that are sufficient to promote reporter gene expression in the developing zebrafish nervous system are not sufficient to promote expression in regenerating retinal ganglion cells in zebrafish. Recently, we identified a 3.6-kb gap43 promoter fragment from the pufferfish, Takifugu rubripes (fugu), that can promote reporter gene expression during both development and regeneration. Using promoter deletion analysis, we have found regions of the 3.6-kb fugu gap43 promoter that are necessary for expression in regenerating, but not developing, retinal ganglion cells. Within the 3.6-kb promoter, we have identified elements that are highly conserved among fish, as well as elements conserved among fish, mammals, and birds.
Author List
Kusik BW, Hammond DR, Udvadia AJAuthor
Ava Udvadia BS,PhD Associate Professor in the Biological Sciences department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Genetically Modified
Base Sequence
Conserved Sequence
GAP-43 Protein
Gene Expression Regulation, Developmental
Molecular Sequence Data
Promoter Regions, Genetic
Regeneration
Retina
Retinal Ganglion Cells
Transgenes
Zebrafish
