Faculty Collaboration Database

Subunits of IgM reconstitute defective contact sensitivity in B-1 cell-deficient xid mice: kappa light chains recruit T cells independent of complement. J Immunol 2002 Oct 15;169(8):4113-23

Date

10/09/2002

Pubmed ID

12370339

DOI

10.4049/jimmunol.169.8.4113

Scopus ID

2-s2.0-0037108379 (requires institutional sign-in at Scopus site)   27 Citations

Abstract

The elicitation of contact sensitivity (CS) to local skin challenge with the hapten trinitrophenyl (TNP) chloride requires an early process that is necessary for local recruitment of CS-effector T cells. This is called CS initiation and is due to the B-1 subset of B cells activated at immunization to produce circulating IgM Ab. At challenge, the IgM binds hapten Ag in a complex that locally activates C to generate C5a that aids in T cell recruitment. In this study, we present evidence that CS initiation is indeed mediated by C-activating classic IgM anti-TNP pentamer. We further demonstrate the involvement of IgM subunits derived either from hybridomas or from lymphoid cells of actively immunized mice. Thus, reduced and alkylated anti-TNP IgM also initiates CS, likely due to generated H chain-L chain dimers, as does a mixture of separated H and L chains that still could weakly bind hapten, but could not activate C. Remarkably, anti-TNP kappa L chains alone mediated CS initiation that was C-independent, but was dependent on mast cells. Thus, B-1 cell-mediated CS initiation required for T cell recruitment is due to activation of C by specific IgM pentamer, and also subunits of IgM, while kappa L chains act via another C-independent but mast cell-dependent pathway.

Author List

Paliwal V, Tsuji RF, Szczepanik M, Kawikova I, Campos RA, Kneilling M, Röcken M, Schuurman J, Redegeld FA, Nijkamp FP, Askenase PW

Author

Vipin Paliwal PhD Associate Professor in the Physics & Chemistry department at Milwaukee School of Engineering

MESH terms used to index this publication - Major topics in bold

Animals
B-Lymphocyte Subsets
Binding Sites, Antibody
Complement Activation
Complement System Proteins
Dermatitis, Contact
Dimerization
Ear, External
Edema
Female
Haptens
Immunoglobulin Heavy Chains
Immunoglobulin M
Immunoglobulin kappa-Chains
Injections, Intravenous
Lymphopenia
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred DBA
Mice, Mutant Strains
Protein Subunits
T-Lymphocyte Subsets
Trinitrobenzenes