Adoptive cell transfer of contact sensitivity-initiation mediated by nonimmune cells sensitized with monoclonal IgE antibodies. Dependence on host skin mast cells. J Immunol 1995 May 15;154(10):5080-92
Date
05/15/1995Pubmed ID
7730614Scopus ID
2-s2.0-0029039389 (requires institutional sign-in at Scopus site) 18 CitationsAbstract
A role for mast cell release of serotonin (5-HT), via Ag-specific factors derived from Thy-1+ B220+ lymphoid cells in the initiation of murine contact sensitivity (CS) has been suggested. However, because CS in mast cell-deficient mice was intact, a role for mast cells in CS initiation was unclear. Therefore, we examined whether CS could be initiated by i.v. injection of nonimmune mixed lymphoid cells that were sensitized in vitro with IgE. When naive mice received IgE-sensitized nonimmune spleen or lymph node cells, or IgE-sensitized purified mast cells, together with immune CS-effector B220- T cells, which therefore were depleted of CS-initiating, Thy-1+, B220+ cells, which could not transfer CS, then reconstitution of CS occurred. Mast cell-deficient W/Wv mice could not elicit this IgE-dependent CS ear swelling, but when mast cell deficiency was reversed by ear injection of normal bone marrow-derived cultured mast cells, then CS was restored. In vitro pretreatment with irrelevant monoclonal anti-OVA IgE prevented CS initiation mediated by Ag-specific, IgE mAb-sensitized cells, presumably by blocking sensitization with IgE. Thus Fc epsilon R on the normal lymphoid cells were involved. When ketanserin, a 5-HT2 receptor antagonist, was injected i.v. before cell transfer, CS initiation via IgE-sensitized cells and CS were no longer elicited. Thus, in this system, IgE Abs bound to circulating IgE Fc epsilon R bearing lymphoid cells sensitized in vitro (most likely basophils), probably mediated early activation of these circulating basophils to release mediators, causing 5-HT release from cutaneous mast cells, to mediate CS initiation.
Author List
Matsuda H, Ushio H, Paliwal V, Ptak W, Askenase PWAuthor
Vipin Paliwal PhD Associate Professor in the Physics & Chemistry department at Milwaukee School of EngineeringMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies, Monoclonal
Bone Marrow Cells
Cell Movement
Cells, Cultured
Dermatitis, Contact
Immunoglobulin E
Ketanserin
Male
Mast Cells
Mice
Mice, Inbred BALB C
Mice, Inbred CBA
Mice, Mutant Strains
Serotonin
Skin
