Faculty Collaboration Database

Urinary proteome analysis in patients with stable SFU grade 4 ureteropelvic junction obstruction differs from normal. Urology 2013 Sep;82(3):745.e1-10

Date

08/31/2013

Pubmed ID

23987180

DOI

10.1016/j.urology.2013.06.009

Scopus ID

2-s2.0-84883258381 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

OBJECTIVE: To evaluate and analyze the urinary proteome in infants with stable grade 4 ureteropelvic junction obstruction (UPJO) and compare to age-matched normal controls.

METHODS: Bladder urine specimens were obtained from 21 healthy infants with normal maternal/fetal ultrasound and 25 infants with grade 4 unilateral UPJO. All patients had >40% ipsilateral individual kidney function by renal scanning and the anteroposterior (AP) diameter of the hydronephrotic kidney ranged from 1.6-3.9 cms at presentation. Over a 5-year follow-up period, the disease progressed in 7 infants (28%), resolved in 4 (16%), and remains stable in the majority (56%). The urinary specimens were prepared using standard methods and subjected to LC/MS/MS analysis. The normalized data were annotated utilizing the Ingenuity Pathways Analysis (IPA; www.Ingenuity.com) knowledge platform.

RESULTS: In the stable UPJO group, the urinary proteomes obtained in infancy differed significantly from the age-matched controls. Analysis revealed important differences in a number of biologic functions including inflammation, apoptosis, tubular injury and fibrosis, and reactive oxygen species response.

CONCLUSION: The urinary proteomes from the bladder in patients with stable grade 4 UPJO (by imaging criteria) are significantly different at birth and during the first year of life and seem to indicate the presence of an ongoing active renal response to UPJO. The imminent discovery of surrogate urinary biomarkers may result in reconsideration of the watchful waiting strategy during this critical period of renal maturation and development in infancy.

Author List

Mesrobian HG, Kryger JV, Groth TW, Fiscus GE, Mirza SP

Author

John V. Kryger MD Chief, Professor in the Urologic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Biomarkers
Case-Control Studies
Chromatography, Liquid
Disease Progression
Female
Follow-Up Studies
Humans
Hydronephrosis
Infant
Infant, Newborn
Inflammation
Male
Mass Spectrometry
Proteome
Ureteral Obstruction