Honokiol produces anti-neoplastic effects on melanoma cells in vitro. J Surg Oncol 2011 Sep 01;104(3):260-4
Date
04/08/2011Pubmed ID
21472732DOI
10.1002/jso.21936Scopus ID
2-s2.0-80051552550 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
BACKGROUND: Melanoma continues to be a therapeutic challenge for the medical community owing to the scarcity of effective agents available to treat the disease. Honokiol, a traditional Chinese herb, has been proven to have anti-cancer effects in various cell types, therefore we hypothesized it may have similar cytotoxic capabilities against melanoma cells in vitro.
METHODS: Two cell lines, SK-MEL2 and MeWo, were grown in culture and exposed to increasing doses of Honokiol. Cell proliferation, cytochrome c release into the cytosol, intra-cellular caspase activity, and mitochondrial depolarization were then evaluated after treatment with honokiol.
RESULTS: Melanoma cells in culture underwent cell death, had increased cytosolic cytochrome c, showed greater caspase activity, and demonstrated increased mitochondrial depolarization after treatment when compared to controls.
CONCLUSIONS: It appears that honokiol is an effective inhibitor of cultured human melanoma cells.
Author List
Mannal PW, Schneider J, Tangada A, McDonald D, McFadden DWAuthor
John G. Schneider MD Assistant Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antineoplastic Agents, PhytogenicApoptosis
Biphenyl Compounds
Blotting, Western
Caspases
Cell Proliferation
Cytochromes c
Drugs, Chinese Herbal
Enzyme-Linked Immunosorbent Assay
Humans
In Vitro Techniques
Lignans
Melanoma
Tumor Cells, Cultured
