Faculty Collaboration Database

Evidence for frequency-dependent arterial damage in vibrated rat tails. Anat Rec A Discov Mol Cell Evol Biol 2005 Jun;284(2):511-21

Date

03/26/2005

Pubmed ID

15791580

DOI

10.1002/ar.a.20186

Scopus ID

2-s2.0-20144366348 (requires institutional sign-in at Scopus site)   51 Citations

Abstract

The effects of single 4-hr bouts of continuous 30, 60, 120, and 800 Hz tail vibration (49 m/sec2, root mean squared) were compared to assess frequency-amplitude-related structural damage of the ventral caudal artery. Amplitudes were 3.9, 0.98, 0.24, and 0.0055 mm, respectively. Vibrated, sham-vibrated, and normal arteries were processed for light and electron microscopy. The Curry rat tail model of hand-arm vibration (Curry et al. Muscle Nerve 2002;25:527-534) proved well-suited for testing multiple frequencies. NFATc3 immunostaining, an early marker of cell damage, increased in smooth muscle and endothelial cells after 30, 60, and 120 Hz but not 800 Hz. Increased vacuolization, which is indicative of smooth muscle contraction, occurred for all frequencies except 800 Hz. Vacuoles increased in both endothelial and smooth muscle cells after 60 and 120 Hz. Only 30 Hz showed pronounced smooth muscle cell vacuolization along the internal and external elastic membranes, suggesting stretch-mediated contraction from the large amplitude shear stress. Discontinuities in toluidine blue staining of the internal elastic membrane (IEM) increased for all frequencies, indicating vibration-induced structural weakening of this structure. Patches of missing IEM and overlying endothelium occurred in approximately 5% of arteries after 60, 120, and 800 Hz. The pattern of damage after 800 Hz suggests that the IEM is disrupted because it resonates at this frequency. Vibration acceleration stress and smooth muscle contraction appear to be the major contributors to arterial damage. The pattern of vibration-induced arterial damage of smooth muscle and endothelial cells is frequency-amplitude-dependent.

Author List

Curry BD, Govindaraju SR, Bain JL, Zhang LL, Yan JG, Matloub HS, Riley DA

Author

Hani S. Matloub MD Professor in the Plastic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Arteries
DNA-Binding Proteins
Endothelium, Vascular
Immunohistochemistry
Male
NFATC Transcription Factors
Occupational Diseases
Physical Stimulation
Rats
Rats, Sprague-Dawley
Regional Blood Flow
Restraint, Physical
Tail
Transcription Factors
Vibration