Estimating in vitro mitochondrial oxygen consumption during muscle contraction and recovery: a novel approach that accounts for diffusion. Ann Biomed Eng 2005 Mar;33(3):343-55
Date
05/05/2005Pubmed ID
15868725DOI
10.1007/s10439-005-1737-7Scopus ID
2-s2.0-17444368348 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
A deconvolution algorithm, based on a Bayesian statistical framework and smoothing spline technique, is applied to reconstructing input functions from noisy measurements in biological systems. Deconvolution is usually ill-posed. However, placing a Bayesian prior distribution on the input function can make the problem well-posed. Using this algorithm and a computational model of diffusional oxygen transport in an approximately cylindrical muscle (about 0.5-mm diameter and 10-mm long mouse leg muscle), the time course of muscle oxygen uptake and mitochondrial oxygen consumption, both during isometric twitch contractions (at various frequencies) and the recovery period, is estimated from polarographic measurements of oxygen concentration on the muscle surface. An important feature of our experimental protocol is the availability of data for the apparatus characteristics. From these time courses, the actual mitochondrial consumption rates during resting and exercise states can be estimated. Mitochondrial oxygen consumption rate increased during stimulation to a maximum steady state value approximately five times of the resting value of 0.63 nmol/s/g wet weight for the stimulation conditions studied. Diffusion slowed the kinetic responses to the contraction but not the steady state fluxes during the stimulation interval.
Author List
Dash RK, Bell BM, Kushmerick MJ, Vicini PAuthor
Ranjan K. Dash PhD Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adaptation, PhysiologicalAnimals
Computer Simulation
In Vitro Techniques
Mice
Mitochondria, Muscle
Models, Biological
Muscle Contraction
Muscle, Skeletal
Oxygen
Oxygen Consumption
