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The human Penumbra gene is mapped to a region on chromosome 7 frequently deleted in myeloid malignancies. Cancer Genet Cytogenet 2005 Oct 15;162(2):95-8

Date

10/11/2005

Pubmed ID

16213355

DOI

10.1016/j.cancergencyto.2005.03.017

Abstract

We previously cloned the murine Penumbra gene based on its differential expression in proerythroblasts/erythroblasts. Subsequently, we identified human Penumbra cDNA from a human bone marrow cDNA library and the human Penumbra gene from a BAC library. Penumbra is a new member of the tetraspanin protein family and exhibits growth-suppressive activity in vitro. In this study, we designed a human Penumbra probe contig and used fluorescent in situ hybridization (FISH) to analyze seven cases of myeloid malignancies with 7q deletions. Five patients with cytogenetic deletions involving 7q31.2 approximately q32 also showed deletions of Penumbra by FISH; these were not present in two patients with cytogenetic deletions not involving 7q31.2 approximately q32. Our findings provide the first FISH evidence supporting the mapping of human Penumbra to 7q31.2 approximately q32 and demonstrate the potential of the Penumbra probe in the detection of 7q31 approximately q32-related deletions in myeloid malignancies.

Author List

Chen Z, Pasquini M, Hong B, DeHart S, Heikens M, Tsai S

Author

Marcelo C. Pasquini MD, MS Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adult
Aged
Chromosome Deletion
Chromosomes, Human, Pair 7
Female
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia, Myeloid, Acute
Male
Membrane Proteins
Middle Aged
Myelodysplastic Syndromes
Tetraspanins
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a