Faculty Collaboration Database

Histochemical staining of nerve endings as an aid to free muscle transplantation. Microsurgery 1991;12(5):361-6

Date

01/01/1991

Pubmed ID

1722864

DOI

10.1002/micr.1920120508

Scopus ID

2-s2.0-0025985452 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

Histochemical staining techniques that identify intact motor nerve fascicles are available to aid free muscle transplantation. Cholinesterase activity of myelinated axons can be identified by Karnovsky and Roots's technique. Axon viability can be assessed based on the presence of axoplasmic enzyme activity. By reacting serial sections for cholinesterase activity and carbonic anhydrase activity, which labels sensory axons, an accurate cross-sectional map of regenerating or functional sensory and motor nerve fibers can be constructed. Resolving the motor and sensory identities of fascicles in a mixed peripheral nerve should lead to more precise coaptation of recipient motor fibers to the motor nerve of the transferred muscle and enhance reinnervation.

Author List

Sanger JR, Riley DA, Yousif NJ, Matloub HS, Bain JL

Authors

Hani S. Matloub MD Professor in the Plastic Surgery department at Medical College of Wisconsin
James R. Sanger MD Professor in the Plastic Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Axilla
Axons
Carbonic Anhydrases
Cholinesterases
Facial Nerve
Facial Paralysis
Humans
Male
Motor Neurons
Muscles
Nerve Endings
Nerve Fibers, Myelinated
Staining and Labeling
Ulnar Nerve