Ligand competition binding assay for the androgen receptor. Methods Mol Biol 2011;776:59-68
Date
07/29/2011Pubmed ID
21796520DOI
10.1007/978-1-61779-243-4_4Scopus ID
2-s2.0-80052952709 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.
Author List
Féau C, Arnold LA, Kosinski A, Guy RKAuthor
Alexander (Leggy) Arnold PhD Professor in the Chemistry & Biochemistry department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
Binding, CompetitiveEscherichia coli
Humans
Ligands
Male
Radioligand Assay
Receptors, Androgen
Recombinant Proteins
