Faculty Collaboration Database

Immunotoxicology of cigarette smoke condensates: suppression of macrophage responsiveness to interferon gamma. Toxicol Appl Pharmacol 1998 Apr;149(2):136-43

Date

05/08/1998

Pubmed ID

9571981

DOI

10.1006/taap.1997.8346

Scopus ID

2-s2.0-0032053589 (requires institutional sign-in at Scopus site)   27 Citations

Abstract

We have investigated systematically the effects of short-term exposure to main stream cigarette smoke condensates (CSC-MS) on basal and inducible functional capacities of murine peritoneal exudate macrophages. Macrophages treated with CSC-MS form granules that fluoresce orange under blue excitation, consistent with the speculation that they are polycyclic aromatic hydrocarbons (PAH). CSC-MS selectively suppressed interferon gamma (IFN gamma) induction of four macrophage functional capacities: enhanced phagocytosis of immunoglobulin-opsonized sheep red blood cells, TPA-induced H2O2 production, class II major histocompatibility complex expression, and nitric oxide synthesis. In contrast, two macrophage functions that are not induced by IFN gamma, basal electron transport and LPS-induced TNF alpha production, were enhanced by treatment with CSC-MS. These results suggest that the suppressive effects of CSC-MS on macrophage responsiveness were selective and were not due to nonspecific inhibition of general functions such as RNA or protein synthesis. Since macrophage responsiveness to IFN gamma can result in induction of functional capacities that are fundamental to immunity, the data suggest that CSC-MS maybe deleterious to the general health of the smoker.

Author List

Braun KM, Cornish T, Valm A, Cundiff J, Pauly JL, Fan S

Author

Toby Charles Cornish MD, PhD Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Carcinogens
Electron Transport
Female
Histocompatibility Antigens Class II
Immunity, Cellular
Interferon-gamma
Lipopolysaccharides
Macrophages, Peritoneal
Mice
Mice, Inbred C57BL
Nitric Oxide
Phagocytosis
Polycyclic Aromatic Hydrocarbons
Respiratory Burst
Tobacco Smoke Pollution
Tumor Necrosis Factor-alpha