Lens extrusion from Laminin alpha 1 mutant zebrafish. ScientificWorldJournal 2014;2014:524929
Date
02/15/2014Pubmed ID
24526906Pubmed Central ID
PMC3914655DOI
10.1155/2014/524929Scopus ID
2-s2.0-84893841071 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
We report analysis of the ocular lens phenotype of the recessive, larval lethal zebrafish mutant, lama1 (a69/a69). Previous work revealed that this mutant has a shortened body axis and eye defects including a defective hyaloid vasculature, focal corneal dysplasia, and loss of the crystalline lens. While these studies highlight the importance of laminin α1 in lens development, a detailed analysis of the lens defects seen in these mutants was not reported. In the present study, we analyze the lenticular anomalies seen in the lama1 (a69/a69) mutants and show that the lens defects result from the anterior extrusion of lens material from the eye secondary to structural defects in the lens capsule and developing corneal epithelium associated with basement membrane loss. Our analysis provides further insights into the role of the lens capsule and corneal basement membrane in the structural integrity of the developing eye.
Author List
Pathania M, Semina EV, Duncan MKAuthor
Elena V. Semina PhD Chief, Professor in the Ophthalmology and Visual Sciences department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBasement Membrane
Collagen Type IV
Cornea
Laminin
Lens Capsule, Crystalline
Lens, Crystalline
Mutation
Phenotype
Zebrafish
Zebrafish Proteins