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Lens extrusion from Laminin alpha 1 mutant zebrafish. ScientificWorldJournal 2014;2014:524929

Date

02/15/2014

Pubmed ID

24526906

Pubmed Central ID

PMC3914655

DOI

10.1155/2014/524929

Scopus ID

2-s2.0-84893841071 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

We report analysis of the ocular lens phenotype of the recessive, larval lethal zebrafish mutant, lama1 (a69/a69). Previous work revealed that this mutant has a shortened body axis and eye defects including a defective hyaloid vasculature, focal corneal dysplasia, and loss of the crystalline lens. While these studies highlight the importance of laminin α1 in lens development, a detailed analysis of the lens defects seen in these mutants was not reported. In the present study, we analyze the lenticular anomalies seen in the lama1 (a69/a69) mutants and show that the lens defects result from the anterior extrusion of lens material from the eye secondary to structural defects in the lens capsule and developing corneal epithelium associated with basement membrane loss. Our analysis provides further insights into the role of the lens capsule and corneal basement membrane in the structural integrity of the developing eye.

Author List

Pathania M, Semina EV, Duncan MK

Author

Elena V. Semina PhD Chief, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Basement Membrane
Collagen Type IV
Cornea
Laminin
Lens Capsule, Crystalline
Lens, Crystalline
Mutation
Phenotype
Zebrafish
Zebrafish Proteins