Flow cytometric features of angioimmunoblastic T-cell lymphoma. Cytometry B Clin Cytom 2006 May;70(3):142-8
Date
03/31/2006Pubmed ID
16572417DOI
10.1002/cyto.b.20107Scopus ID
2-s2.0-33646485237 (requires institutional sign-in at Scopus site) 46 CitationsAbstract
BACKGROUND: The immunophenotypic features of angioimmunoblastic T-cell lymphoma (AILT) have not been well described.
METHODS: We retrospectively reviewed our institutional experience with the flow cytometric features of 16 cases of AILT.
RESULTS: Multiparameter flow cytometry was able to identify a distinct population of immunophenotypically aberrant T cells in 15 of 16 cases. In 13 lymph node specimens, the neoplastic cells ranged from 1.9 to 87% (median 23%) of cells. The ratio of reactive to neoplastic T cells ranged from 0.01 to 20 (median 1.5); reactive T cells outnumbered neoplastic in 9/13 (69%) cases. The neoplastic populations expressed CD2, CD4, CD5, and CD45RO in all cases, lacked expression of CD8 and CD56 in all cases, and showed negative or dim surface CD3 in most cases. CD10 was expressed by the neoplastic populations in 11 of 14 cases at diagnosis; in 3 of these 11 only a subpopulation of the neoplastic cells was CD10(+). CD10 tended to be absent on neoplastic cells in staging bone marrows. The neoplastic population in all but one of the 15 positive cases possessed multiple immunophenotypic abnormalities and these were generally retained during the follow-up analyses of several cases.
CONCLUSIONS: These results indicate the potential utility of flow cytometry in the diagnosis and follow-up of AILT.
Author List
Chen W, Kesler MV, Karandikar NJ, McKenna RW, Kroft SHAuthor
Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAged
Aged, 80 and over
Antigens, CD
Antigens, CD7
Bone Marrow
CD3 Complex
Female
Flow Cytometry
Follow-Up Studies
Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
Humans
Immunoblastic Lymphadenopathy
Immunophenotyping
Leukocyte Common Antigens
Lymph Nodes
Lymphoma, T-Cell
Male
Middle Aged
Neprilysin
Receptors, Complement 3d
Retrospective Studies
T-Lymphocytes
