Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. NMR Biomed 1993;6(1):89-94
Date
01/01/1993Pubmed ID
8384470DOI
10.1002/nbm.1940060114Scopus ID
2-s2.0-0027345896 (requires institutional sign-in at Scopus site) 280 CitationsAbstract
The use of cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated. The method is validated on standard samples of N-acetyl aspartate (NAA) and lactate, and possible sources of error are discussed. Using the STEAM pulse sequence, concentrations of choline, creatine and NAA in frontal lobe white matter are found to be 1.9 +/- 0.5, 10.6 +/- 1.3 and 16.6 +/- 2.3 mumol/g wet wt, respectively, in 10 normal volunteers. In the thalamus, the concentrations are 2.0 +/- 0.4, 11.6 +/- 2.0 and 17.2 +/- 1.3 mumol/g wet wt, respectively. Choline and creatine concentrations are in good agreement with conventional biochemical values: NAA concentrations are found to be three-fold higher, suggesting overlap of the NAA signal with other compounds. Quantitation relative to tissue water is a convenient and rapid means of quantitating proton spectra of the human brain.
Author List
Barker PB, Soher BJ, Blackband SJ, Chatham JC, Mathews VP, Bryan RNAuthor
Vincent Mathews MD Chair, Professor in the Radiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultAspartic Acid
Brain Chemistry
Choline
Creatine
Female
Humans
Magnetic Resonance Spectroscopy
Male
Middle Aged
Protons
Reference Standards
Reproducibility of Results
Water
