Linkage disequilibrium mapping in domestic dog breeds narrows the progressive rod-cone degeneration interval and identifies ancestral disease-transmitting chromosome. Genomics 2006 Nov;88(5):541-50
Date
07/25/2006Pubmed ID
16859891Pubmed Central ID
PMC4006154DOI
10.1016/j.ygeno.2006.05.013Scopus ID
2-s2.0-33749430640 (requires institutional sign-in at Scopus site) 61 CitationsAbstract
Canine progressive rod-cone degeneration (prcd) is a retinal disease previously mapped to a broad, gene-rich centromeric region of canine chromosome 9. As allelic disorders are present in multiple breeds, we used linkage disequilibrium (LD) to narrow the approximately 6.4-Mb interval candidate region. Multiple dog breeds, each representing genetically isolated populations, were typed for SNPs and other polymorphisms identified from BACs. The candidate region was initially localized to a 1.5-Mb zero recombination interval between growth factor receptor-bound protein 2 (GRB2) and SEC14-like 1 (SEC14L). A fine-scale haplotype of the region was developed, which reduced the LD interval to 106 kb and identified a conserved haplotype of 98 polymorphisms present in all prcd-affected chromosomes from 14 different dog breeds. The findings strongly suggest that a common ancestor transmitted the prcd disease allele to many of the modern dog breeds and demonstrate the power of the LD approach in the canine model.
Author List
Goldstein O, Zangerl B, Pearce-Kelling S, Sidjanin DJ, Kijas JW, Felix J, Acland GM, Aguirre GDAuthor
Danielle Sidjanin Maier PhD Nurse Practitioner in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBase Sequence
Breeding
Chromosome Mapping
Chromosomes, Artificial, Bacterial
DNA, Complementary
Dog Diseases
Dogs
Female
Genetic Predisposition to Disease
Genetics, Population
Genomics
Haplotypes
Linkage Disequilibrium
Male
Phylogeny
Retinal Degeneration
Species Specificity
