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Intestinal alkaline phosphatase is protective to the preterm rat pup intestine. J Pediatr Surg 2014 Jun;49(6):954-60; discussion 960

Date

06/04/2014

Pubmed ID

24888842

Pubmed Central ID

PMC4130394

DOI

10.1016/j.jpedsurg.2014.01.031

Scopus ID

2-s2.0-84901801080   13 Citations

Abstract

BACKGROUND: Necrotizing enterocolitis (NEC) is the most common surgical emergency in neonates, with a mortality rate between 10 and 50%. The onset of necrotizing enterocolitis is highly variable and associated with numerous risk factors. Prior research has shown that enteral supplementation with intestinal alkaline phosphatase (IAP) decreases the severity of NEC. The aim of this study is to investigate whether IAP is protective to the preterm intestine in the presence of formula feeding and in the absence of NEC.

METHODS: Preterm rat pups were fed formula with or without supplementation with IAP, and intestine was obtained on day of life 3 for analysis of IAP activity, mRNA expression of TNFα, IL-6 and iNOS and permeability and cytokine expression after LPS exposure.

RESULTS: There was no difference in the absolute and intestine specific alkaline phosphatase activity in both groups. Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFα, IL-6 and iNOS. Pups supplemented with IAP had decreased permeability and inflammatory cytokine expression after exposure to LPS ex vivo when compared to formula fed controls.

CONCLUSIONS: Our results support that IAP is beneficial to preterm intestine and decreases intestinal injury and inflammation caused by LPS.

Author List

Heinzerling NP, Liedel JL, Welak SR, Fredrich K, Biesterveld BE, Pritchard KA Jr, Gourlay DM

Authors

David M. Gourlay MD Chief, Professor in the Surgery department at Medical College of Wisconsin
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin
Scott R. Welak MD Assistant Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Administration, Oral
Alkaline Phosphatase
Animals
Animals, Newborn
Cytokines
Disease Models, Animal
Enterocolitis, Necrotizing
Gene Expression Regulation, Developmental
Intestinal Mucosa
RNA, Messenger
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a