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Quantitative assessment of intestinal injury using a novel in vivo, near-infrared imaging technique. Mol Imaging 2010 Feb;9(1):30-9

Date

02/05/2010

Pubmed ID

20128996

Pubmed Central ID

PMC4241240

DOI

10.2310/7290.2010.00001

Scopus ID

2-s2.0-76749112573   13 Citations

Abstract

Intestinal injury owing to inflammation, severe trauma, and burn is a leading cause of morbidity and mortality. Currently, animal models employed to study the intestinal response to injury and inflammation depend on outdated methods of analysis. Given that these classic intestinal assays are lethal to the experimental animal, there is no ability to study the gut response to injury in the same animal over time. We postulated that by developing an in vivo assay to image intestinal injury using fluorescent dye, it could complement other expensive, time-consuming, and semiquantitative classic means of detecting intestinal injury. We describe a novel in vivo, noninvasive method to image intestinal injury using a charge-coupled device (CCD) camera that allows for serial visual and quantitative analysis of intestinal injury. Our results correlate with traditional, time-consuming, semiquantitative assays of intestinal injury, now allowing the noninvasive, nonlethal assessment of injury over time.

Author List

Costantini TW, Eliceiri BP, Peterson CY, Loomis WH, Putnam JG, Baird A, Wolf P, Bansal V, Coimbra R

Author

Carrie Peterson MD, MS, FACS, FASCRS Associate Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Burns
Dextrans
Disease Models, Animal
Fluorescein-5-isothiocyanate
Fluorometry
Histocytochemistry
Intestinal Mucosa
Intestines
Male
Mice
Mice, Inbred BALB C
Spectroscopy, Near-Infrared
Statistics, Nonparametric
Whole Body Imaging