Decreased HIV type 1 transcription in CCR5-Δ32 heterozygotes during suppressive antiretroviral therapy. J Infect Dis 2014 Dec 01;210(11):1838-43
Date
06/18/2014Pubmed ID
24935955Pubmed Central ID
PMC4271057DOI
10.1093/infdis/jiu338Scopus ID
2-s2.0-84922277880 (requires institutional sign-in at Scopus site) 10 CitationsAbstract
Individuals who are heterozygous for the CCR5-Δ32 mutation provide a natural model to examine the effects of reduced CCR5 expression on human immunodeficiency virus (HIV) persistence. We evaluated the HIV reservoir in 18 CCR5-Δ32 heterozygotes and 54 CCR5 wild-type individuals during suppressive antiretroviral therapy. Cell-associated HIV RNA levels (P=.035), RNA to DNA transcriptional ratios (P=.013), and frequency of detectable HIV 2-long terminal repeat circular DNA (P=.013) were significantly lower in CD4+ T cells from CCR5-Δ32 heterozygotes. Cell-associated HIV RNA was significantly correlated with CCR5 surface expression on CD4+ T cells (r2=0.136; P=.002). Our findings suggest that curative strategies should further explore manipulation of CCR5.
Author List
Wang C, Abdel-Mohsen M, Strain MC, Lada SM, Yukl S, Cockerham LR, Pilcher CD, Hecht FM, Sinclair E, Liegler T, Richman DD, Deeks SG, Pillai SKAuthor
Leslie Cockerham MD Associate Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Antiretroviral Therapy, Highly ActiveCD4 Lymphocyte Count
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Cohort Studies
Gene Expression Regulation, Viral
Genotype
HIV Infections
HIV-1
Heterozygote
Humans
Mutation
Phenotype
RNA, Viral
Receptors, CCR5
Viral Load
