Faculty Collaboration Database

Small bowel transplantation promotes bacterial overgrowth and translocation. J Surg Res 1991 Dec;51(6):512-7

Date

12/01/1991

Pubmed ID

1943089

DOI

10.1016/0022-4804(91)90174-k

Scopus ID

2-s2.0-0026316970 (requires institutional sign-in at Scopus site)   32 Citations

Abstract

Alterations in the symbiotic relationship between immunocompromised hosts and their resident gut microflora may lead to serious complications following small bowel transplantation (SBT). This study examined the effects of SBT and cyclosporine (CsA) immunosuppression on gut bacterial populations and translocation to the mesenteric lymph nodes. Sixty adult male meat-fed Lewis rats were divided into six groups: normal controls, CsA alone (24 mg/kg im qod), CsA carrier vehicle alone, isografts, isografts given CsA, and allografts given CsA. Rats were killed after 3 weeks and segments of small bowel and colon were harvested for quantitative tissue culture. Mesenteric lymph nodes and blood were cultured to identify translocation. Transplantation alone led to an increase in gram-negative aerobes from 2.6 to 4.6 colony forming units/100 mg tissue (P less than 0.05) in the distal ileum (transplanted segment). Eighty-four percent of transplanted animals receiving CsA had bacteria recovered from their mesenteric lymph nodes compared to none in controls (P less than 0.001) and 20% in isografts not receiving CsA (P less than 0.02). Intestinal transplantation alone appears to promote gram-negative overgrowth while the addition of CsA therapy facilitates translocation to the mesenteric lymph nodes and may predispose to gut-associated sepsis following SBT.

Author List

Browne BJ, Johnson CP, Edmiston CE, Hlava MA, Moore GH, Roza AM, Telford GL, Adams MB

Author

Christopher P. Johnson MD Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Bacterial Physiological Phenomena
Blood
Cell Movement
Colon
Colony Count, Microbial
Cyclosporine
Intestine, Small
Lymph Nodes
Male
Mesentery
Peritoneum
Rats
Rats, Inbred Strains