Faculty Collaboration Database

Isoprostane levels in lipids extracted from atherosclerotic arteries of nonhuman primates. Free Radic Biol Med 2001 Jun 15;30(12):1337-46

Date

06/08/2001

Pubmed ID

11390178

DOI

10.1016/s0891-5849(01)00527-5

Scopus ID

2-s2.0-0035877154 (requires institutional sign-in at Scopus site)   11 Citations

Abstract

Nonhuman primates used in these studies had been fed for 5 years diets enriched with cholesterol and one of three classes of fatty acids: saturated, monounsaturated, or polyunsaturated fatty acids. Atherosclerotic iliac artery lipid extracts were quantitatively analyzed for cholesterol, cholesteryl esters, fatty acid composition, and a marker of lipid oxidation, the F(2)-isoprostanes. There was no significant difference in the mean accumulation of F(2)-isoprostanes among the different diet groups. To account for the small, individual variation in the arachidonate concentration the F(2)-isoprostane mass from each sample was normalized by dividing by arachidonate mass: F(2)-isoprostane mass/(mass arachidonate). At lower levels of cholesterol accumulation, the F(2)-isoprostane mass/(mass arachidonate) ratio was greater in lipids from POLY arteries compared to SAT arteries, but the reverse was true at high levels of cholesterol. F(2)-isoprostane/(mass arachidonate) increased with mole fraction linoleate for the SAT group, but decreased for the POLY group. In summary, these studies demonstrated that there is no simple explanation of how F(2)-isoprostane accumulation did not depend on the concentration of oxidizable lipids that promote free-radical lipid oxidation.

Author List

Thomas MJ, Chen Q, Sorci-Thomas MG, Rudel LL

Authors

Mary Sorci Thomas PhD Professor in the Medicine department at Medical College of Wisconsin
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Arachidonic Acids
Arteriosclerosis
Cholesterol
Cholesterol Esters
Cholesterol, LDL
Diet, Atherogenic
Dietary Fats
Dinoprost
Fatty Acids
Free Radicals
Iliac Artery
Linoleic Acid
Lipid Peroxidation
Lipids
Oleic Acid
Oxidation-Reduction
Palm Oil
Plant Oils
Safflower Oil