Platelet-activating factor. Methods Enzymol 2007;434:105-16
Date
10/24/2007Pubmed ID
17954244DOI
10.1016/S0076-6879(07)34006-8Scopus ID
2-s2.0-38449114847 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Platelet-activating factor (PAF) is a potent mediator that occurs at very low concentrations in cells and tissues. Accurate quantitation of PAF has always been difficult because of the physicochemical properties of PAF and its structural similarity to several much more abundant phospholipids. Numerous assays for PAF have been developed, all of which have their strengths and limitations. Herein, this chapter describes a high-pressure liquid chromatography (HPLC)-tandem mass spectrometry assay for PAF. Major strengths of the method are its sensitivity (detection limit = 1 pg) and selectivity. Another advantage is that, by using liquid instead of gas chromatography, sample derivatization is avoided. The limitations of the method are its use of expensive instrumentation and the requirement of performing two HPLC runs per sample. Detailed technical advice on application of the method to various types of samples is given.
Author List
Owen JS, Thomas MJ, Wykle RLAuthor
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CalibrationChromatography, High Pressure Liquid
Deuterium
Gas Chromatography-Mass Spectrometry
Indicators and Reagents
Isotope Labeling
Platelet Activating Factor
Spectrometry, Mass, Electrospray Ionization
