Anaplastic large cell lymphoma: a flow cytometric analysis of 29 cases. Am J Clin Pathol 2007 Aug;128(2):314-22
Date
07/20/2007Pubmed ID
17638668DOI
10.1309/GUHKGAJEJ72CEAL7Scopus ID
2-s2.0-34547886159 (requires institutional sign-in at Scopus site) 54 CitationsAbstract
We report our experience with flow cytometric (FC) analysis of 29 cases of anaplastic large cell lymphoma (ALCL). Morphologic analysis of processed cytocentrifuged preparations demonstrated neoplastic cells in 28 cases. In 25 of these, an aberrant lymphoid population was detected by FC analysis. The majority showed high orthogonal light scatter, similar to monocytes or granulocytes. Of the antigens CD2, CD3, CD4, CD5, and CD7, 5 cases expressed 1, 8 expressed 2, 6 expressed 3, 3 expressed 4, and 3 expressed all 5. CD4 was expressed most commonly (20/25 [80%]), followed by CD2 (18/25 [72%]), CD3 (10/25 [40%]), and CD5 and CD7 (8/25 [32%] each). CD45 was expressed in 23 of 25 cases and CD13 in 7 of 9. Of 21 cases, 13 were anaplastic lymphoma kinase (ALK)+, all of which were CD4+, vs 5 of 8 ALK - cases (P = .042). Most ALCLs can be detected and characterized by multiparameter FC analysis. However, light scatter gating on typical lymphoid regions may yield false-negative results in a substantial number of cases.
Author List
Kesler MV, Paranjape GS, Asplund SL, McKenna RW, Jamal S, Kroft SHAuthor
Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Aged
Antigens, CD
Child
Child, Preschool
Female
Flow Cytometry
Humans
Ki-1 Antigen
Leukocyte Common Antigens
Light
Lymphoma, Large B-Cell, Diffuse
Male
Middle Aged
Protein-Tyrosine Kinases
Receptor Protein-Tyrosine Kinases
Scattering, Radiation