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Anaplastic large cell lymphoma: a flow cytometric analysis of 29 cases. Am J Clin Pathol 2007 Aug;128(2):314-22

Date

07/20/2007

Pubmed ID

17638668

DOI

10.1309/GUHKGAJEJ72CEAL7

Scopus ID

2-s2.0-34547886159 (requires institutional sign-in at Scopus site)   54 Citations

Abstract

We report our experience with flow cytometric (FC) analysis of 29 cases of anaplastic large cell lymphoma (ALCL). Morphologic analysis of processed cytocentrifuged preparations demonstrated neoplastic cells in 28 cases. In 25 of these, an aberrant lymphoid population was detected by FC analysis. The majority showed high orthogonal light scatter, similar to monocytes or granulocytes. Of the antigens CD2, CD3, CD4, CD5, and CD7, 5 cases expressed 1, 8 expressed 2, 6 expressed 3, 3 expressed 4, and 3 expressed all 5. CD4 was expressed most commonly (20/25 [80%]), followed by CD2 (18/25 [72%]), CD3 (10/25 [40%]), and CD5 and CD7 (8/25 [32%] each). CD45 was expressed in 23 of 25 cases and CD13 in 7 of 9. Of 21 cases, 13 were anaplastic lymphoma kinase (ALK)+, all of which were CD4+, vs 5 of 8 ALK - cases (P = .042). Most ALCLs can be detected and characterized by multiparameter FC analysis. However, light scatter gating on typical lymphoid regions may yield false-negative results in a substantial number of cases.

Author List

Kesler MV, Paranjape GS, Asplund SL, McKenna RW, Jamal S, Kroft SH

Author

Steven Howard Kroft MD Chair, Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adolescent
Adult
Aged
Antigens, CD
Child
Child, Preschool
Female
Flow Cytometry
Humans
Ki-1 Antigen
Leukocyte Common Antigens
Light
Lymphoma, Large B-Cell, Diffuse
Male
Middle Aged
Protein-Tyrosine Kinases
Receptor Protein-Tyrosine Kinases
Scattering, Radiation