5-Oxo-eicosatetraenoate, a potent human neutrophil stimulus. Biochem Biophys Res Commun 1993 Apr 15;192(1):129-34
Date
04/15/1993Pubmed ID
8386504DOI
10.1006/bbrc.1993.1391Scopus ID
2-s2.0-0027236081 (requires institutional sign-in at Scopus site) 53 CitationsAbstract
5-Oxo-eicosatetraenoate (5-oxoETE), a newly defined arachidonate metabolite, resembled 5-hydroxyeicosatetraenoate (5-HETE) in stimulating neutrophils to mobilize Ca2+ an in promoting PMN degranulation responses to other agents. It was, however, 10-fold stronger than 5-HETE and, like leukotriene (LT) B4, had intrinsic PMN degranulating effects. Nonetheless, 5-oxoETE and 5-HETE desensitized PMN to themselves or each other but not to LTB4; LTB4 desensitized to itself but not to 5-oxoETE or 5-HETE; and an antagonist blocked LTB4 but not 5-oxoETE or 5-HETE. 5-OxoETE and 5-HETE thus induce diverse PMN responses using a shared, down-regulatable, and receptor-like mechanism that does not involve LTB4 receptors; 5-oxoETE is the preferred natural agonist for this mechanism.
Author List
O'Flaherty JT, Cordes J, Redman J, Thomas MJAuthor
Michael J. Thomas PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Arachidonic AcidsCalcium
Cell Degranulation
Cells, Cultured
Humans
Hydroxyeicosatetraenoic Acids
In Vitro Techniques
Leukotriene B4
Neutrophils
Tetrazoles
