Gene expression. MicroRNA control of protein expression noise. Science 2015 Apr 03;348(6230):128-32
Date
04/04/2015Pubmed ID
25838385DOI
10.1126/science.aaa1738Scopus ID
2-s2.0-84928754032 (requires institutional sign-in at Scopus site) 293 CitationsAbstract
MicroRNAs (miRNAs) repress the expression of many genes in metazoans by accelerating messenger RNA degradation and inhibiting translation, thereby reducing the level of protein. However, miRNAs only slightly reduce the mean expression of most targeted proteins, leading to speculation about their role in the variability, or noise, of protein expression. We used mathematical modeling and single-cell reporter assays to show that miRNAs, in conjunction with increased transcription, decrease protein expression noise for lowly expressed genes but increase noise for highly expressed genes. Genes that are regulated by multiple miRNAs show more-pronounced noise reduction. We estimate that hundreds of (lowly expressed) genes in mouse embryonic stem cells have reduced noise due to substantial miRNA regulation. Our findings suggest that miRNAs confer precision to protein expression and thus offer plausible explanations for the commonly observed combinatorial targeting of endogenous genes by multiple miRNAs, as well as the preferential targeting of lowly expressed genes.
Author List
Schmiedel JM, Klemm SL, Zheng Y, Sahay A, Blüthgen N, Marks DS, van Oudenaarden AAuthor
David S. Marks MD Vice Chair, Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
3' Untranslated RegionsAnimals
Embryonic Stem Cells
Gene Expression Regulation
Mice
MicroRNAs
Models, Genetic
Protein Biosynthesis
RNA, Messenger
Single-Cell Analysis
Transcription, Genetic