Faculty Collaboration Database

Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows. Analyst 2015 Jun 21;140(12):4046-56

Date

05/23/2015

Pubmed ID

26000346

Pubmed Central ID

PMC4536072

DOI

10.1039/c5an00729a

Scopus ID

2-s2.0-84930722181 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

The chemical status of live sensory neurons is accessible with infrared microspectroscopy of appropriately prepared cells. In this paper, individual dorsal root ganglion (DRG) neurons have been prepared with two different protocols, and plated on glass cover slips, BaF2 and CaF2 substrates. The first protocol exposes the intact DRGs to 4 °C for between 20-30 minutes before dissociating individual neurons and plating 2 hours later. The second protocol maintains the neurons at 23 °C for the entire duration of the sample preparation. The visual appearance of the neurons is similar. The viability was assessed by means of trypan blue exclusion method to determine the viability of the neurons. The neurons prepared under the first protocol (cold exposure) and plated on BaF2 reveal a distinct chemical signature and chemical distribution that is different from the other sample preparations described in the paper. Importantly, results for other sample preparation methods, using various substrates and temperature protocols, when compared across the overlapping spectral bandwidth, present normal chemical distribution within the neurons. The unusual chemically specific spatial variation is dominated by a lack of protein and carbohydrates in the center of the neurons and signatures of unraveling DNA are detected. We suggest that cold shock leads to apoptosis of DRGs, followed by osmotic stress originating from ion gradients across the cell membrane leading to cell lysis.

Author List

Aboualizadeh E, Mattson EC, O'Hara CL, Smith AK, Stucky CL, Hirschmugl CJ

Author

Cheryl L. Stucky PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Apoptosis
Calcium Fluoride
Cold-Shock Response
Ganglia, Spinal
Infrared Rays
Male
Mice
Mice, Inbred C57BL
Neurons
Principal Component Analysis
Spectroscopy, Fourier Transform Infrared