Comparison of Peripheral Nerve Axonal Area Differences in Central and Peripheral Zones of Injured and Repaired Nerves. J Reconstr Microsurg 2015 Oct;31(8):551-7
Date
07/01/2015Pubmed ID
26125148DOI
10.1055/s-0035-1555140Scopus ID
2-s2.0-84941642150 (requires institutional sign-in at Scopus site)Abstract
BACKGROUND: Histological analysis remains a cornerstone approach for the investigation of peripheral nerve regeneration. This study investigates a newly recognized histological difference between peripheral and central zones within the regenerating nerve trunks.
PURPOSE: The purpose of the study was to determine if the nerve axonal area (NXA) in regenerating peripheral nerves differs within central and peripheral areas, when viewed in cross-section.
METHODS: A total of 14 rats were divided into two groups, and subjected to different injuries to the right sciatic nerve. Group 1: Transection injury with immediate repair. Group 2: Crush injury without any treatment. The left sciatic nerve was left uninjured and served as a control in each rat. Following 4 weeks of recovery, nerve trunk cross-sections were prepared. Computerized techniques were then employed to divide nerve sections into central and peripheral zones and calculate corresponding NXA values for subsequent statistical analysis.
RESULTS: NXA of injured nerves was greater within peripheral as compared with the central zones, independent of injury type (p < 0.05). No statistically significant difference existed within the control groups or between the injury methods with regards to NXA regeneration extent.
CONCLUSION: NXA in regenerating peripheral nerves was greater in the peripheral zones than within the central zones.
Author List
Thayer JA, Yan JG, Zhang LL, Havlik R, Agresti MAuthor
Robert Havlik MD Chair, Professor in the Plastic Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAxons
Biopsy, Needle
Disease Models, Animal
Immunohistochemistry
Male
Nerve Regeneration
Neurosurgical Procedures
Peripheral Nerve Injuries
Random Allocation
Rats
Rats, Sprague-Dawley
Risk Assessment
Sciatic Nerve
Treatment Outcome
