Vessel microport technique for applications in cerebrovascular research. J Neurosci Res 2009 May 15;87(7):1718-27
Date
12/31/2008Pubmed ID
19115415Pubmed Central ID
PMC2713928DOI
10.1002/jnr.21973Scopus ID
2-s2.0-65849265212 (requires institutional sign-in at Scopus site) 33 CitationsAbstract
Cerebrovascular research suffers from a lack of reliable methods with which to deliver exogenous substances effectively into the central nervous system (CNS) of small experimental animals. Here we describe a novel vessel microport surgical technique for a variety of cerebrovascular applications that is reproducible and well tolerated in mice. The procedure is based on the insertion of a vessel microport into the external carotid artery for substance delivery into the CNS via the internal carotid artery. The method results in selective substance delivery into the ipsilateral hemisphere. Other novel aspects of this surgical technique include the ability to perform multiple injections, study of conscious mice well removed from surgery, and lack of occlusion of the common or internal carotid artery that allows carotid flow to be maintained. The feasibility of this technique has been validated by infusion of HIV Tat protein to induce permeability of the blood-brain barrier and by implantation of tumor cells to establish a brain metastasis model. Thus, the described vessel microport technique can be employed in a variety of cerebrovascular research applications.
Author List
Chen L, Swartz KR, Toborek MAuthor
Karin R. Swartz MD Assistant Dean, Professor in the Neurosurgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBlood-Brain Barrier
Brain
Brain Neoplasms
Carotid Artery, Internal
Cerebrovascular Circulation
Disease Models, Animal
Drug Delivery Systems
Mice
Mice, Inbred C57BL
Microvessels
Neoplasm Metastasis
Soot
tat Gene Products, Human Immunodeficiency Virus
