Faculty Collaboration Database

Changes in [125I]hCGRP binding in rat spinal cord in an experimental model of acute, peripheral inflammation. Brain Res 1992 Sep 25;591(2):198-208

Date

09/25/1992

Pubmed ID

1332803

DOI

10.1016/0006-8993(92)91700-o

Scopus ID

2-s2.0-0026616801 (requires institutional sign-in at Scopus site)   21 Citations

Abstract

[125I]Human calcitonin gene-related peptide ([125I]hCGRP) binding in the dorsal horn of the spinal cord exhibited differential changes among laminae over time in response to unilateral adjuvant-induced inflammation. In laminae I/II, 4 days after induction of inflammation, the binding decreased 36% on the side of the spinal cord ipsilateral to the inflammation, while there was no change on the contralateral side. The decrease ipsilateral to inflammation was due primarily to a decrease in the Bmax of the high affinity binding site for CGRP. In lamina V, the binding increased 18% on both sides of the spinal cord at the same time point. In lamina X, the binding increased 16% on both sides of the spinal cord at 2 days after induction of inflammation and remained increased at 8 days. The increases in [125]hCGRP binding in laminae V and X were primarily due to a decrease in the Kd of the low affinity binding site for CGRP. the accompanying hyperalgesia was first measured at 2 days after induction of inflammation and persisted at 8 days. Because the changes in [125I]hCGRP binding did not parallel the hyperalgesia accompanying the unilateral adjuvant-induced inflammation, we believe that CGRP receptors are not directly involved with the hyperalgesia but may be involved with other plastic changes observed in the spinal cord during unilateral adjuvant-induced inflammation.

Author List

Galeazza MT, Stucky CL, Seybold VS

Author

Cheryl L. Stucky PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Acute Disease
Animals
Behavior, Animal
Calcitonin Gene-Related Peptide
Disease Models, Animal
Humans
Hyperalgesia
Inflammation
Iodine Radioisotopes
Male
Nerve Fibers
Neurons, Afferent
Peripheral Nervous System Diseases
Radioligand Assay
Rats
Rats, Sprague-Dawley
Receptors, Calcitonin
Receptors, Cell Surface
Spinal Cord