Faculty Collaboration Database

The effect of duodenal-jejunal bypass on glucose-dependent insulinotropic polypeptide secretion in Wistar rats. Obes Surg 2010 Jun;20(6):768-75

Date

02/24/2010

Pubmed ID

20177809

Pubmed Central ID

PMC3761799

DOI

10.1007/s11695-010-0095-1

Scopus ID

2-s2.0-77953229019 (requires institutional sign-in at Scopus site)   11 Citations

Abstract

BACKGROUND: Enteroendocrine K cells secrete the incretin hormone glucose-dependent insulinotropic peptide (GIP) and are predominately located in the duodenum. GIP levels should decrease after gastric bypass due to duodenal exclusion; however, studies have found conflicting data regarding the changes in GIP secretion after gastric bypass and duodenal-jejunal bypass (DJB).

METHODS: We performed a DJB or Sham surgery on Wistar rats followed by an oral glucose tolerance test on postoperative (post-op) day 12 and superior mesenteric lymphatic cannulation on post-op day 14. We measured meal-stimulated GIP concentrations and small bowel GIP and GLP-1 protein content after DJB or Sham surgery.

RESULTS: There was no difference in glucose tolerance by 12 days post-op. We found no difference in lymphatic GIP concentration area under the curve between DJB and Sham rats (15,240 pg/ml min +/- 2,651 vs. 17,201 pg/ml min +/- 2,763, respectively, p = 0.62). GIP and GLP-1 protein contents were both significantly increased only in the midjejunum in DJB rats compared to Sham rats (p = 0.009 and p = 0.01, respectively).

CONCLUSIONS: Plasma and lymphatic GIP concentrations did not significantly change after DJB in Wistar rats. DJB increased GIP protein content in the midjejunum at the new site of nutrient absorption, but this was surprisingly not countered by a decrease in GIP protein content in the bypassed duodenum. Further studies are needed to determine the mechanisms that account for the discrepancy in GIP production and subsequent secretion after DJB as well as what role GIP plays in the effect of gastrointestinal surgery on glucose homeostasis.

Author List

Kindel TL, Yoder SM, D'Alessio DA, Tso P

Author

Tammy Lyn Kindel MD, PhD Associate Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blood Glucose
Disease Models, Animal
Duodenum
Enteroendocrine Cells
Gastric Bypass
Gastric Inhibitory Polypeptide
Glucose Tolerance Test
Insulin
Jejunum
Male
Rats
Rats, Wistar
Treatment Outcome