Glomerular beta-galactosidase expression following transduction with microsphere-adenoviral complexes. Kidney Int 2002 Jan;61(1 Suppl):S68-72
Date
02/14/2002Pubmed ID
11841616DOI
10.1046/j.1523-1755.2002.0610s1068.xScopus ID
2-s2.0-0036175763 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
The aortic injection of adenoviral-microsphere complexes is a useful technique for in vivo gene transfer (transduction) to the glomerulus. In this approach, the appearance of the foreign transprotein in the glomerulus may result from glomerular cell gene transfer and local synthesis or hepatic cell transduction followed by synthesis, secretion, and deposition in the glomerulus. We postulated that glomerular expression of the foreign transgene was the result of glomerular cell transduction. To test this question, male SD rats underwent aortic injections with adenovirus containing the LacZ expression cassette [expressing beta-galactosidase (betagal)] coupled to 16 microm diameter microspheres. After 48 hours, histologic staining confirmed glomerular expression of the betagal transprotein and reverse transcription in situ polymerase chain reaction demonstrated the presence of the betagal transgene in the glomerulus. In addition, hepatic expression of the betagal transprotein was minimal and substantially less than that observed in the glomeruli. These data support the contention that adenoviral-microsphere complexes result in glomerular cell transduction with the desired transgene, followed by local transprotein synthesis. This approach may prove useful for facilitating glomerular gene transfer in the development of gene therapy for glomerulonephritis.
Author List
Bhatt UY, Sferra TJ, Johnson A, Williams C, Shirey K, Venema T, Nuovo GJ, Nahman NS JrAuthor
Tammy Lyn Kindel MD, PhD Associate Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdenoviridaeAnimals
Kidney Glomerulus
Male
Microspheres
Rats
Rats, Sprague-Dawley
Transduction, Genetic
beta-Galactosidase
