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Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiae. Genetics 2008 Nov;180(3):1391-405



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Scopus ID

2-s2.0-59449110397 (requires institutional sign-in at Scopus site)   5 Citations


Nonsense-mediated mRNA decay (NMD) performs two functions in eukaryotes, one in controlling the expression level of a substantial subset of genes and the other in RNA surveillance. In the vast majority of genes, nonsense mutations render the corresponding transcripts prone to surveillance and subject to rapid degradation by NMD. To examine whether some classes of nonsense transcripts escape surveillance, we asked whether NMD acts on mRNAs that undergo subcellular localization prior to translation. In Saccharomyces cerevisiae, wild-type ASH1 mRNA is one of several dozen transcripts that are exported from the mother-cell nucleus during mitotic anaphase, transported to the bud tip on actin cables, anchored at the bud tip, and translated. Although repressed during transport, translation is a prerequisite for NMD. We found that ash1 nonsense mutations affect transport and/or anchoring independently of NMD. The nonsense transcripts respond to NMD in a manner dependent on the position of the mutation. Maximal sensitivity to NMD occurs when transport and translational repression are simultaneously impaired. Overall, our results suggest a model in which ash1 mRNAs are insensitive to NMD while translation is repressed during transport but become sensitive once repression is relieved.

Author List

Zheng W, Finkel JS, Landers SM, Long RM, Culbertson MR


Roy M. Long PhD Assistant Dean, Associate Professor in the Medical School Regional Campuses department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Biological Transport
Blotting, Northern
Cell Nucleus
Codon, Nonsense
DNA-Binding Proteins
Fluorescent Dyes
Gene Expression Regulation, Fungal
Open Reading Frames
Polymerase Chain Reaction
RNA Processing, Post-Transcriptional
RNA Stability
RNA, Fungal
RNA, Messenger
Repressor Proteins
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription, Genetic